HomeCU DenverCollege of Liberal Arts & ScienceCLAS LabsDepartment of Integrative Biology

Reagents

GSK-3α Plasmids

Plasmid C-term V5 tag   N-term GST tag   pCS2+   mCherry tag   pCR8GW fusion   pCR8GW stop
FL GSK-3α ORF X X X X X X
GSK-3α ΔNT X X X X X X
GSK-3α ΔCT1 X X X X X
GSK-3α ΔCT2 X X X X X X
GSK-3α ΔCT3 X X X X X
GSK-3α ΔCT4 X X X X X
GSK-3α P442/443A X X X X
GSK-3α K148M X X X X
GSK-3α K148R X X X X X X
GSK-3α S21A X X X X
GSK-3α R159A X X X X X
GSK-3α Y279F X X X X X

GSK-3β Plasmids

Plasmid C-term V5 tag   N-term GST tag   pCS2+   mCherry tag   pCR8GW fusion   pCR8GW stop
FL GSK-3β ORF X X X X X X
GSK-3β ΔNT X X X X
GSK-3β ΔCT1 X X X X X X
GSK-3β ΔCT2 X X X X X X
GSK-3β ΔCT3 X X X X X
GSK-3β ΔCT4 X X X
GSK-3β P379/380A X X X
GSK-3β K85M X X X
GSK-3β K85R X X X X X
GSK-3β S9A X X X X X X
GSK-3β R96A X X X X X X
GSK-3β Y216F X X X X X X

 

Methylation-related Plasmids

  • Dnmt3a2 pCAGEN
  • Dnmt3a2 WT promoter pCR8GW
  • Dnmt3a2 NMyc mut promoter pCR8GW
  • Dnmt3a2 WT promoter pGLF
  • Dnmt3a2 NMyc mut promoter pGLF

Cloning Plasmids

  • pcDNATM-DEST47 (C-terminal GFP tag)
  • pcDNATM-DEST53 (N-terminal GFP tag)
  • pDESTTM26 (N-terminal 6xHis tag)
  • pDESTTM27 (N-terminal GST tag)
  • pBAD-DEST49 (Bacterial Expression)
  • pcDNATM-DEST40 (C-terminal V5 6xHis tag)
  • pCAGEN-GW (chicken β-actin promoter, untagged)
  • pCS2+GW (Gateway® Compatible destination vector)
  • pMACS Kk II-GW (Gateway® Compatible destination vector for use with Miltenyi Biotec magnetic bead enrichment of transfected mammalian cells)
University of Colorado Denver logo

© 2014 The Regents of the University of Colorado, a body corporate. All rights reserved. All trademarks are registered property of the University. Used by permission only.